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Wednesday, October 9, 2024
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Search the Herbicide-Resistance Reference Database
Searches the reference database - authors, title, abstract, and keywords fields. Not case sensitive.
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REFERENCES
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Huang, Z., J. Chen, C. Zhang, H. Huang, S. Wei, X. Zhou, J. Chen, and X. Wang
.
2016
.
Target-site basis for resistance to imazethapyr in redroot amaranth (
Amaranthus retroflexus
L.).
.
Pesticide Biochemistry and Physiology
128
:
10 - 15
.
Experiments were conducted to confirm imazethapyr resistance in redroot amaranth (
Amaranthus retroflexus
L.) and study the target-site based mechanism for the resistance. Whole-plant response experiments revealed that the resistant (R) population exhibited 19.16 fold resistance to imazethapyr compared with the susceptible (S) population.
In vitro
ALS activity assay demonstrated that the imazethapyr I
50
value of the R population was 21.33 times greater than that of the S population. However, qRT-PCR analysis revealed that there is no difference in ALS gene expression between the R and S populations. Sequence analysis revealed an Asp-376-Glu substitution in ALS in the R population. In order to verify that the imazethapyr resistance was conferred by Asp-376-Glu mutation, the
ALS-R
and
ALS-S
genes were fused to the CaMV 35S promoter and introduced into Arabidopsis respectively. The expression of
ALS-R
in transgenic Arabidopsis plants exhibited 13.79 fold resistance to imazethapyr compared to
ALS-S
transgenic Arabidopsis.
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PERMISSION MUST BE OBTAINED FIRST if you intend to base a significant portion of a scientific paper on data derived from this site.
Cite this site as:
Heap, I. The International Survey of Herbicide Resistant Weeds. Online. Internet.
Wednesday, October 9, 2024
. Available
www.weedscience.org
Copyright � 1993-
2024
WeedScience.org All rights reserved. Fair use of this material is encouraged. Proper citation is requested.
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