Search the Herbicide-Resistance Reference Database

Searches the reference database - authors, title, abstract, and keywords fields. Not case sensitive.
You can use AND, OR, AND NOT operators. For instance : Tranel and Amaranthus and not glyphosate

REFERENCES

1

Page size:

select

• 5
• 10
• 20
• 50

Page: of 1

Items 1 to 1 of 1

Cha ThyeSan ; Anne-Marie, K. ; Chuah TseSeng. 2014. Identification and characterization of RAPD-SCAR markers linked to glyphosate-susceptible and -resistant biotypes of Eleusine indica (L.) Gaertn. Molecular Biology Reports 41 : 823 - 831.

Eleusine indica is one of the most common weed species found in agricultural land worldwide. Although herbicide-glyphosate provides good control of the weed, its frequent uses has led to abundant reported cases of resistance. Hence, the development of genetic markers for quick detection of glyphosate-resistance in E. indica population is imperative for the control and management of the weed. In this study, a total of 14 specific random amplified polymorphic DNA (RAPD) markers were identified and two of the markers, namely S4R727 and S26R₆976 were further sequence characterized. Sequence alignment revealed that marker S4R727 showing a 12-bp nucleotides deletion in resistant biotypes, while marker S26R6976 contained a 167-bp nucleotides insertion in the resistant biotypes. Based on these sequence differences, three pairs of new sequence characterized amplified region (SCAR) primers were developed. The specificity of these primer pairs were further validated with genomic DNA extracted from ten individual plants of one glyphosate-susceptible and five glyphosate-resistant (R2, R4, R6, R8 and R11) populations. The resulting RAPD-SCAR markers provided the basis for assessing genetic diversity between glyphosate-susceptible and -resistant E. indica biotypes, as well for the identification of genetic locus link to glyphosate-resistance event in the species.

1

Page size:

select

• 5
• 10
• 20
• 50

Page: of 1

Items 1 to 1 of 1

PERMISSION MUST BE OBTAINED FIRST if you intend to base a significant portion of a scientific paper on data derived from this site.
Cite this site as: Heap, I.  The International Survey of Herbicide Resistant Weeds.  Online.  Internet.  Wednesday, October 9, 2024 .  Available  www.weedscience.org
Copyright � 1993- 2024 WeedScience.org All rights reserved. Fair use of this material is encouraged. Proper citation is requested.